Category Archives: Uncategorized

FlexiBAC, our open source protein expression system

One of great things here at the MPI-CBG are our Service and Facilities, among them the protein expression facility (PEPC), which has been heavily used by us in recent years. In collaboration with Jeff Woodruff, a former postdoc, they developed FlexiBAC, an amazingly versatile protein expression system in insect cells, which was developed with a […]

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We held the first EMBO phase separation course here at the MPI-CBG

From 4th to 13th February we taught our first EMBO course Methods for Studying Phase Separation in Biology here in Dresden at the MPI-CBG and CSBD. The course was organized together with the Tang and Alberti lab, including morning lectures, theoretical or experimental parts during the day and a relaxing evening part. Importantly, we included […]

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Phase separation buffers noise in cells

Now, the paper got published in Science! Congratulations to Adam and all the coauthors. And check out the perspective article about the paper from the Brangwynne lab (@Brangwynnelab), as well as the press article from the CSBD and from the CBG . We are excited to share our pre-print which explores how phase separation affects […]

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Rheology of protein condensates using optical traps

An increasing number of proteins with intrinsically disordered domains have been shown to phase separate in buffer to form liquidlike phases. These protein condensates serve as simple models for the investigation of the more complex membraneless organelles in cells. To understand the function of such proteins in cells, the material properties of the condensates they […]

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How do you choose a research topic?

Choosing a research topic is an important decision at any level. The choice shapes decisions about what graduate lab to join, which post-doctoral position to pursue, how to start an independent lab, and what companies might make good employers. The thing to remember is that “the choice” can, and perhaps should, be made many times […]

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Phase Transitions form Virus Replication Compartments in Cells

Zoltan’s paper is out! Former postdoc Zoltan worked with the Whelan lab on VSV (Vesicular Stomatitis Virus). RNA viruses like VSV compartmentalize their replication machinery to evade detection by the host. However, it has been unclear how the virus can concentrate the machinery for RNA synthesis. Now it is shown that the replication compartments have […]

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Former postdoc Richard opens his own lab in Oxford

Congratulations to Richard! Check out his website

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We are organizing an EMBO Practical Course for studying phase separation in biology!

The course takes place 05 – 13 February 2019 in Dresden at the MPI-CBG, register until 1st Oct. 2018, we are looking forward to seeing you here!   #EMBOphaseseparation / facebook   About the Practical Course The field of cell biology is in the midst of a revolution in the understanding of how cells are […]

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Jie’s paper on the molecular grammar of phase separation is out!

Our latest publication is out now in Cell! Congratulations to Jie and all the co-workers.   In this work, Jie Wang and colleagues worked with a family of prion like proteins (FUS family proteins) and suggest rules that define their phase separation behavior. In cells, FUS family proteins can phase separate into liquid like organelles. However, […]

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Check out our new user’s guide for phase separation assays with purified proteins!

Highlights Membrane-less organelles form by phase separation Membrane-less organelles can be reconstituted from minimal components Phase-separating proteins are difficult to purify and handle We provide guidelines and protocols for working with phase-separating proteins The formation of membrane-less organelles and compartments by protein phase separation is an important way in which cells organize their cytoplasm and […]

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  • Selected Videos

    iBio video about P granule formation

    An interview with Tony about Phase Transitions and Disease

    Phase separation in cell polarity: Saha et al, Cell 2016

    Encouraging Innovation,

    The genetics linking temperature and fertility in worms: Leaver et al, Biology Open 2016

    Cell PaperFlick on Phase Transitions in Disease

    Check out this playlist to watch all the videos in our "Two Minute Talk" Series

    Ways of Growing, a film created for the MitoSys Project

    What is a Discovery?

    Embryonic Development of C.elegans