TransgeneOmics Collaborations

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Publications resulting from collaborations using our HeLa/BAC TransgeneOmics lines 

2016
Antibody validation in bioimaging applications based on endogenous expression of tagged proteins.
Skogs M, Stadler C, Schutten R, Hjelmare M, Gnann C, Björk L, Poser I, Hyman AA, Uhlén M, Lundberg EK.
J Proteome Res. 2016 Oct 11.

Ki-67 acts as a biological surfactant to disperse mitotic chromosomes.
Cuylen S, Blaukopf C, Politi AZ, Müller-Reichert T, Neumann B, Poser I, Ellenberg J, Hyman AA, Gerlich DW.
Nature. 2016 Jun 29;535(7611):308-12. doi: 10.1038/nature18610.

Molecular basis for CPAP-tubulin interaction in controlling centriolar and ciliary length.
Zheng X, Ramani A, Soni K, Gottardo M, Zheng S, Ming Gooi L, Li W, Feng S, Mariappan A, Wason A, Widlund P, Pozniakovsky A, Poser I, Deng H, Ou G, Riparbelli M, Giuliano C, Hyman AA, Sattler M, Gopalakrishnan J, Li H.
Nat Commun. 2016 Jun 16;7:11874. doi: 10.1038/ncomms11874.

PLEKHA7 recruits PDZD11 to adherens junctions to stabilize nectins.
Guerrera D, Shah J, Vasileva E, Sluysmans S, Mean I, Jond L, Poser I, Mann M, Hyman AA, Citi S.
J Biol Chem. 2016 Apr 4. pii: jbc.M115.712935. [Epub ahead of print]

CPAP promotes timely cilium disassembly to maintain neural progenitor pool.
Gabriel E, Wason A, Ramani A, Gooi LM, Keller P, Pozniakovsky A, Poser I, Noack F, Telugu NS, Calegari F, Šarić T, Hescheler J, Hyman AA, Gottardo M, Callaini G, Alkuraya FS, Gopalakrishnan J.
EMBO J. 2016 Apr 15;35(8):803-19.

2015
A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M.
Cell. 2015 Oct 22;163(3):712-23.

Sestrin 2 protein regulates platelet-derived growth factor receptor β (Pdgfrβ) expression by modulating proteasomal and Nrf2 transcription factor functions.
Tomasovic A, Kurrle N, Sürün D, Heidler J, Husnjak K, Poser I, Schnütgen F, Scheibe S, Seimetz M, Jaksch P, Hyman A, Weissmann N, von Melchner H.
J Biol Chem. 2015 Apr 10;290(15):9738-52.

Quantitative comparison of a human cancer cell surface proteome between interphase and mitosis.
Özlü N, Qureshi MH, Toyoda Y, Renard BY, Mollaoglu G, Özkan NE, Bulbul S, Poser I, Timm W, Hyman AA, Mitchison TJ, Steen JA.
EMBO J. 2015 Jan 13;34(2):251-65.

2014
Conserved TCP domain of Sas-4/CPAP is essential for pericentriolar material tethering during centrosome biogenesis.
Zheng X, Gooi LM, Wason A, Gabriel E, Mehrjardi NZ, Yang Q, Zhang X, Debec A, Basiri ML, Avidor-Reiss T, Pozniakovsky A, Poser I, Saric T, Hyman AA, Li H, Gopalakrishnan J.
Proc Natl Acad Sci U S A. 2014 Jan 21;111(3):E354-63.

2013
C11ORF24 is a novel type I membrane protein that cycles between the Golgi apparatus and the plasma membrane in Rab6-positive vesicles.
Fraisier V, Kasri A, Miserey-Lenkei S, Sibarita JB, Nair D, Mayeux A, Bardin S, Toyoda Y, Poser I, Poznyakovskiy A, Goud B, Hyman AA, Dimitrov A.
PLoS One. 2013 Dec 2;8(12):e82223. doi: 10.1371/journal.pone.0082223. eCollection 2013.

The human cap-binding complex is functionally connected to the nuclear RNA exosome.
Andersen PR, Domanski M, Kristiansen MS, Storvall H, Ntini E, Verheggen C, Schein A, Bunkenborg J, Poser I, Hallais M, Sandberg R, Hyman A, LaCava J, Rout MP, Andersen JS, Bertrand E, Jensen TH.
Nat Struct Mol Biol. 2013 Dec;20(12):1367-76.

The C-terminal domain of Brd2 is important for chromatin interaction and regulation of transcription and alternative splicing.
Hnilicová J, Hozeifi S, Stejskalová E, Dušková E, Poser I, Humpolíčková J, Hof M, Staněk D.
Mol Biol Cell. 2013 Nov;24(22):3557-68.

A conserved RhoGAP limits M phase contractility and coordinates with microtubule asters to confine RhoA during cytokinesis.
Zanin E, Desai A, Poser I, Toyoda Y, Andree C, Moebius C, Bickle M, Conradt B, Piekny A, Oegema K.
Dev Cell. 2013 Sep 16;26(5):496-510.

Aurora B and Kif2A control microtubule length for assembly of a functional central spindle during anaphase.
Uehara R, Tsukada Y, Kamasaki T, Poser I, Yoda K, Gerlich DW, Goshima G.
J Cell Biol. 2013 Aug 19;202(4):623-36.

Centrobin regulates centrosome function in interphase cells by limiting pericentriolar matrix recruitment.
Jeffery JM, Grigoriev I, Poser I, van der Horst A, Hamilton N, Waterhouse N, Bleier J, Subramaniam VN, Maly IV, Akhmanova A, Khanna KK.
Cell Cycle. 2013 Mar 15;12(6):899-906.

A genomic toolkit to investigate kinesin and myosin motor function in cells.
Maliga Z, Junqueira M, Toyoda Y, Ettinger A, Mora-Bermúdez F, Klemm RW, Vasilj A, Guhr E, Ibarlucea-Benitez I, Poser I, Bonifacio E, Huttner WB, Shevchenko A, Hyman AA.
Nat Cell Biol. 2013 Mar;15(3):325-34.

A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility.
Bassik MC, Kampmann M, Lebbink RJ, Wang S, Hein MY, Poser I, Weibezahn J, Horlbeck MA, Chen S, Mann M, Hyman AA, Leproust EM, McManus MT, Weissman JS.
Cell. 2013 Feb 14;152(4):909-22.

Stoichiometry of chromatin-associated protein complexes revealed by label-free quantitative mass spectrometry-based proteomics.
Smits AH, Jansen PW, Poser I, Hyman AA, Vermeulen M.
Nucleic Acids Res. 2013 Jan 7;41(1):e28.

2012
APC15 mediates CDC20 autoubiquitylation by APC/C(MCC) and disassembly of the mitotic checkpoint complex.
Uzunova K, Dye BT, Schutz H, Ladurner R, Petzold G, Toyoda Y, Jarvis MA, Brown NG, Poser I, Novatchkova M, Mechtler K, Hyman AA, Stark H, Schulman BA, Peters JM.
Nat Struct Mol Biol. 2012 Sep 24.

BICD2, dynactin and LIS1 cooperate in regulating dynein recruitment to cellular structures.
Splinter D, Razafsky DS, Schlager MA, Serra-Marques A, Grigoriev I, Demmers J, Keijzer N, Jiang K, Poser I, Hyman AA, Hoogenraad CC, King SJ, Akhmanova A.
Mol Biol Cell. 2012 Sep 5.

Sds22 and Repo-Man stabilize chromosome segregation by counteracting Aurora B on anaphase kinetochores.
Wurzenberger C, Held M, Lampson MA, Poser I, Hyman AA, Gerlich DW.
J Cell Biol. 2012 Jul 23;198(2):173-83.

Functional repurposing revealed by comparing S. pombe and S. cerevisiae genetic interactions.
Frost A, Elgort MG, Brandman O, Ives C, Collins SR, Miller-Vedam L, Weibezahn J, Hein MY, Poser I, Mann M, Hyman AA, Weissman JS.
Cell. 2012 Jun 8;149(6):1339-52.

ARF6 GTPase protects the post-mitotic midbody from 14-3-3-mediated disintegration.
Joseph N, Hutterer A, Poser I, Mishima M.
EMBO J. 2012 May 11;31(11):2604-14.

2011
High-efficiency counterselection recombineering for site-directed mutagenesis in bacterial artificial chromosomes.
Bird AW, Erler A, Fu J, Hériché JK, Maresca M, Zhang Y, Hyman AA, Stewart AF.
Nat Methods. 2011 Dec 4;9(1):103-9.

Systematic phosphorylation analysis of human mitotic protein complexes.
Hegemann B, Hutchins JR, Hudecz O, Novatchkova M, Rameseder J, Sykora MM, Liu S, Mazanek M, Lénárt P, Hériché JK, Poser I, Kraut N, Hyman AA, Yaffe MB, Mechtler K, Peters JM.
Sci Signal. 2011 Nov 8;4(198):rs12.

Large-scale De Novo Prediction of Physical Protein-Protein Association.
Elefsinioti A, Saraç OS, Hegele A, Plake C, Hubner NC, Poser I, Sarov M, Hyman A, Mann M, Schroeder M, Stelzl U, Beyer A.
Mol Cell Proteomics. 2011 Nov;10(11):M111.010629.

Proliferating versus differentiating stem and cancer cells exhibit distinct midbody-release behaviour.
Ettinger AW, Wilsch-Bräuninger M, Marzesco AM, Bickle M, Lohmann A, Maliga Z, Karbanová J, Corbeil D, Hyman AA, Huttner WB.
Nat Commun. 2011 Oct 18;2:503.

From RNAi Screens to Molecular Function in Embryonic Stem Cells.
Ding L, Poser I, Paszkowski-Rogacz M, Buchholz F.
Stem Cell Rev. 2011 Apr 28.

Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods.
Jakobsen L, Vanselow K, Skogs M, Toyoda Y, Lundberg E, Poser I, Falkenby LG, Bennetzen M, Westendorf J, Nigg EA, Uhlen M, Hyman AA, Andersen JS.
EMBO J. 2011 Apr 20;30(8):1520-35.

Cortical constriction during abscission involves helices of ESCRT-III-dependent filaments.
Guizetti J, Schermelleh L, Mäntler J, Maar S, Poser I, Leonhardt H, Müller-Reichert T, Gerlich DW.
Science. 2011 Mar 25;331(6024):1616-20.

Resources for proteomics in mouse embryonic stem cells.
Schnütgen F, Ehrmann F, Poser I, Hubner NC, Hansen J, Floss T, deVries I, Wurst W, Hyman A, Mann M, von Melchner H.
Nat Methods. 2011 Feb;8(2):103-4.

2010
The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells.
Huranová M, Ivani I, Benda A, Poser I, Brody Y, Hof M, Shav-Tal Y, Neugebauer KM, Stanek D.
J Cell Biol. 2010 Oct 4;191(1):75-86.

Live-cell imaging RNAi screen identifies PP2A-B55alpha and importin-beta1 as key mitotic exit regulators in human cells.
Schmitz MH, Held M, Janssens V, Hutchins JR, Hudecz O, Ivanova E, Goris J, Trinkle-Mulcahy L, Lamond AI, Poser I, Hyman AA, Mechtler K, Peters JM, Gerlich DW.
Nat Cell Biol. 2010 Sep;12(9):886-93. Epub 2010 Aug 15.

Global analysis reveals SRp20- and SRp75-specific mRNPs in cycling and neural cells.
Ankö ML, Morales L, Henry I, Beyer A, Neugebauer KM.
Nat Struct Mol Biol. 2010 Aug;17(8):962-70. Epub 2010 Jul 18.

A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia.
Słabicki M, Theis M, Krastev DB, Samsonov S, Mundwiller E, Junqueira M, Paszkowski-Rogacz M, Teyra J, Heninger AK, Poser I, Prieur F, Truchetto J, Confavreux C, Marelli C, Durr A, Camdessanche JP, Brice A, Shevchenko A, Pisabarro MT, Stevanin G, Buchholz F.
PLoS Biol. 2010 Jun 29;8(6):e1000408.

CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex.
Spruijt CG, Bartels SJ, Brinkman AB, Tjeertes JV, Poser I, Stunnenberg HG, Vermeulen M.
Mol Biosyst. 2010 Sep;6(9):1700-6. Epub 2010 Jun 4.

Quantitative proteomics combined with BAC TransgeneOmics reveals in vivo protein interactions.
Hubner NC, Bird AW, Cox J, Splettstoesser B, Bandilla P, Poser I, Hyman A, Mann M.
J Cell Biol. 2010 May 17;189(4):739-54.

Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes.
Neumann B, Walter T, Hériché JK, Bulkescher J, Erfle H, Conrad C, Rogers P, Poser I, Held M, Liebel U, Cetin C, Sieckmann F, Pau G, Kabbe R, Wünsche A, Satagopam V, Schmitz MH, Chapuis C, Gerlich DW, Schneider R, Eils R, Huber W, Peters JM, Hyman AA, Durbin R, Pepperkok R, Ellenberg J.
Nature. 2010 Apr 1;464(7289):721-7.

Systematic analysis of human protein complexes identifies chromosome segregation proteins.
Hutchins JR, Toyoda Y, Hegemann B, Poser I, Hériché JK, Sykora MM, Augsburg M, Hudecz O, Buschhorn BA, Bulkescher J, Conrad C, Comartin D, Schleiffer A, Sarov M, Pozniakovsky A, Slabicki MM, Schloissnig S, Steinmacher I, Leuschner M, Ssykor A, Lawo S, Pelletier L, Stark H, Nasmyth K, Ellenberg J, Durbin R, Buchholz F, Mechtler K, Hyman AA, Peters JM.
Science. 2010 Apr 30;328(5978):593-9. Epub 2010 Apr 1.

Re-examination of siRNA specificity questions role of PICH and Tao1 in the spindle checkpoint and identifies Mad2 as a sensitive target for small RNAs.
Hübner NC, Wang LH, Kaulich M, Descombes P, Poser I, Nigg EA.
Chromosoma. 2010 Apr;119(2):149-65. Epub 2009 Nov 11.

2009
HAUS, the 8-subunit human Augmin complex, regulates centrosome and spindle integrity.
Lawo S, Bashkurov M, Mullin M, Ferreria MG, Kittler R, Habermann B, Tagliaferro A, Poser I, Hutchins JR, Hegemann B, Pinchev D, Buchholz F, Peters JM, Hyman AA, Gingras AC, Pelletier L.
Curr Biol. 2009 May 26;19(10):816-26. Epub 2009 May 7.

SR protein family members display diverse activities in the formation of nascent and mature mRNPs in vivo.
Sapra AK, Ankö ML, Grishina I, Lorenz M, Pabis M, Poser I, Rollins J, Weiland EM, Neugebauer KM.
Mol Cell. 2009 Apr 24;34(2):179-90.

Comparative profiling identifies C13orf3 as a component of the Ska complex required for mammalian cell division.
Theis M, Slabicki M, Junqueira M, Paszkowski-Rogacz M, Sontheimer J, Kittler R, Heninger AK, Glatter T, Kruusmaa K, Poser I, Hyman AA, Pisabarro MT, Gstaiger M, Aebersold R, Shevchenko A, Buchholz F.
EMBO J. 2009 May 20;28(10):1453-65.

A genome-scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity.
Ding L, Paszkowski-Rogacz M, Nitzsche A, Slabicki MM, Heninger AK, de Vries I, Kittler R, Junqueira M, Shevchenko A, Schulz H, Hubner N, Doss MX, Sachinidis A, Hescheler J, Iacone R, Anastassiadis K, Stewart AF, Pisabarro MT, Caldarelli A, Poser I, Theis M, Buchholz F.
Cell Stem Cell. 2009 May 8;4(5):403-15.

2008
Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A.
Bird AW, Hyman AA.
J Cell Biol. 2008 Jul 28;182(2):289-300.

BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals.
Poser I, Sarov M, Hutchins JR, Hériché JK, Toyoda Y, Pozniakovsky A, Weigl D, Nitzsche A, Hegemann B, Bird AW, Pelletier L, Kittler R, Hua S, Naumann R, Augsburg M, Sykora MM, Hofemeister H, Zhang Y, Nasmyth K, White KP, Dietzel S, Mechtler K, Durbin R, Stewart AF, Peters JM, Buchholz F, Hyman AA.
Nat Methods. 2008 May;5(5):409-15.