Selected Publications (since 2003)

Reviews

Howard J, Hyman AA. Growth, fluctuation and switching at microtubule plus ends.
Nat Rev Mol Cell Biol. 2009 Jun 10.
Cowan CR, Hyman AA. Acto-myosin reorganization and PAR polarity in C. elegans.
Development. 2007 Mar;134(6):1035-43. PDF
Howard J, Hyman AA. Microtubule polymerases and depolymerases.
Curr Opin Cell Biol. 2007 Feb;19(1):31-5. PDF
Oegema K, Hyman AA. Cell division.
WormBook. 2006 Jan 19:1-40. WormBook
Kinoshita K, Noetzel TL, Arnal I, Drechsel DN, Hyman AA. Global and local control of microtubule destabilization promoted by a catastrophe kinesin MCAK/XKCM1.
J Muscle Res Cell Motil. 2006;27(2):107-14. PDF
Hyman AA, Howard J. Cell structure and dynamics.
Curr Opin Cell Biol. 2005 Feb;17(1):1-2. PDF
Cowan CR, Hyman AA. Asymmetric cell division in C. elegans: cortical polarity and spindle positioning.
Annu Rev Cell Dev Biol. 2004;20:427-53. PDF
Howard J and Hyman AA. Dynamics and mechanics of the microtubule plus end.
Nature 2003, 422 (6933): 753-8 PDF
Wittmann T, Hyman AA, Desai A. The spindle: a dynamic assembly of microtubules and motors.
Nat Cell Biol. 2001 E28-34 PDF

Microtubule Dynamics

Greenan G, Brangwynne CP, Jaensch S, Gharakhani J, Jülicher F, Hyman AA. Centrosome size sets mitotic spindle length in Caenorhabditis elegans embryos.
Curr Biol. 2010 Feb 23;20(4):353-8. Epub 2010 Feb 4.
Zanic M, Stear JH, Hyman AA, Howard J. EB1 recognizes the nucleotide state of tubulin in the microtubule lattice.
PLoS One. 2009 Oct 23;4(10):e7585. PDF
Lundin VF, Srayko M, Hyman AA, Leroux MR. Efficient chaperone-mediated tubulin biogenesis is essential for cell division and cell migration in C. elegans.
Dev Biol. 2008 Jan 1;313(1):320-34. PDF
Brouhard GJ, Stear JH, Noetzel TL, Al-Bassam J, Kinoshita K, Harrison SC, Howard J, Hyman AA. XMAP215 is a processive microtubule polymerase.
Cell 2008 Jan 11;132(1):79-88. PDF
Elie-Caille C, Severin F, Helenius J, Howard J, Muller DJ, Hyman AA. Straight GDP-tubulin protofilaments form in the presence of taxol.
Curr Biol. 2007 Oct 23;17(20):1765-70. PDF
Al-Bassam J, Larsen NA, Hyman AA, Harrison SC. Crystal structure of a TOG domain: conserved features of XMAP215/Dis1-family TOG domains and implications for tubulin binding.
Structure. 2007 Mar;15(3):355-62. PDF
Howard J, Hyman AA. Microtubule polymerases and depolymerases.
Curr Opin Cell Biol. 2007 Feb;19(1):31-5.
Srayko M, O'toole ET, Hyman AA, Müller-Reichert T. Katanin disrupts the microtubule lattice and increases polymer number in C. elegans meiosis.
Curr Biol. 2006 Oct 10;16(19):1944-9.
Varga V, Helenius J, Tanaka K, Hyman AA, Tanaka TU, Howard J. Yeast kinesin-8 depolymerizes microtubules in a length-dependent manner.
Nat Cell Biol. 2006 Sep;8(9):957-62. PDF
Al-Bassam J, van Breugel M, Harrison SC, Hyman AA. Stu2p binds tubulin and undergoes an open-to-closed conformational change.
J Cell Biol. 2006 Mar 27;172(7):1009-22. PDF
Barros TP, Kinoshita K, Hyman AA, Raff JW. Aurora A activates D-TACC-Msps complexes exclusively at centrosomes to stabilize centrosomal microtubules.
J Cell Biol. 2005 Sep 26;170(7):1039-46. PDF
Srayko M, Kaya A, Stamford J, Hyman AA. Identification and Characterization of Factors Required for Microtubule Growth and Nucleation in the Early C. elegans Embryo.
Dev Cell. 2005 Aug;9(2):223-36. PDF
Noetzel TL, Drechsel DN, Hyman AA, Kinoshita K. A comparison of the ability of XMAP215 and tau to inhibit the microtubule destabilizing activity of XKCM1.
Philos Trans R Soc Lond B Biol Sci. 2005 Mar 29;360(1455):591-4. PDF
Van Breugel M, Hyman AA, Drechsel D. Stu2p, the budding yeast member of the conserved Dis1/XMAP215 family of microtubule-associated proteins is a plus end-binding microtubule destabilizer.
J Cell Biol. 2003 April 28; 161 (2): 1-13 PDF
Howard J and Hyman AA. Dynamics and mechanics of the microtubule plus end.
Nature 2003, 422 (6933): 753-8 PDF
Srayko M, Quintin S, Schwager A, Hyman AA. Caenorhabditis elegans TAC-1 and ZYG-9 form a complex that is essential for long astral and spindle microtubules.
Curr Biol. 2003 Sep 2;13(17):1506-11 PDF
Kinoshita K, Arnal I, Desai A, Drechsel DN, Hyman AA. Reconstitution of Physiological Microtubule Dynamics Using Purified Components.
Science. 2001 294: 1340-1343 PDF
Arnal I, Karsenti E, Hyman AA. Structural Transitions at microtubule ends correlate with their dynamic properties in Xenopus egg extracts.
J Cell Biol. 2000 149: 767-774 PDF

Mitosis

Neumann B, Walter T, Hériché JK, Bulkescher J, Erfle H, Conrad C, Rogers P, Poser I, Held M, Liebel U, Cetin C, Sieckmann F, Pau G, Kabbe R, Wünsche A, Satagopam V, Schmitz MH, Chapuis C, Gerlich DW, Schneider R, Eils R, Huber W, Peters JM, Hyman AA, Durbin R, Pepperkok R, Ellenberg J. Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes.
Nature. 2010 Apr 1;464(7289):721-7.
Hutchins JR, Toyoda Y, Hegemann B, Poser I, Hériché JK, Sykora MM, Augsburg M, Hudecz O, Buschhorn BA, Bulkescher J, Conrad C, Comartin D, Schleiffer A, Sarov M, Pozniakovsky A, Slabicki MM, Schloissnig S, Steinmacher I, Leuschner M, Ssykor A, Lawo S, Pelletier L, Stark H, Nasmyth K, Ellenberg J, Durbin R, Buchholz F, Mechtler K, Hyman AA, Peters JM. Systematic Localization and Purification of Human Protein Complexes Identifies Chromosome Segregation Proteins.
Science. 2010 Apr 1.
Lawo S, Bashkurov M, Mullin M, Ferreria MG, Kittler R, Habermann B, Tagliaferro A, Poser I, Hutchins JR, Hegemann B, Pinchev D, Buchholz F, Peters JM, Hyman AA, Gingras AC, Pelletier L. HAUS, the 8-subunit human Augmin complex, regulates centrosome and spindle integrity.
Curr Biol. 2009 May 26;19(10):816-26.
Bird AW, Hyman AA. Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A.
J Cell Biol. 2008 Jul 28;182(2):289-300. PDF
Zhu F, Lawo S, Bird A, Pinchev D, Ralph A, Richter C, Müller-Reichert T, Kittler R, Hyman AA, Pelletier L. The mammalian SPD-2 ortholog Cep192 regulates centrosome biogenesis.
Curr Biol. 2008 Jan 22;18(2):136-41. PDF
Stirling PC, Srayko M, Takhar KS, Pozniakovsky A, Hyman AA, Leroux MR. Functional interaction between phosducin-like protein 2 and cytosolic chaperonin is essential for cytoskeletal protein function and cell cycle progression.
Mol Biol Cell. 2007 Jun;18(6):2336-45. PDF
Schlaitz AL, Srayko M, Dammermann A, Quintin S, Wielsch N, MacLeod I, de Robillard Q, Zinke A, Yates III JR, Müller-Reichert T, Shevchenko A, Oegema K, Hyman AA. The C. elegans RSA Complex Localizes Protein Phosphatase 2A to Centrosomes and Regulates Mitotic Spindle Assembly.
Cell 2007, January 12; 128(1):115-127.
Pelletier L, O'Toole E, Schwager A, Hyman AA, Müller-Reichert T. Centriole assembly in Caenorhabditis elegans.
Nature 2006 Nov 30;444(7119):619-23. PDF
Kinoshita K, Noetzel TL, Pelletier L, Mechtler K, Drechsel DN, Schwager A, Lee M, Raff JW, Hyman AA. Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis.
J Cell Biol. 2005 Sep 26;170(7):1047-55. PDF
Ozlu N, Srayko M, Kinoshita K, Habermann B, O'toole ET, Muller-Reichert T, Schmalz N, Desai A, Hyman AA. An Essential Function of the C. elegans Ortholog of TPX2 Is to Localize Activated Aurora A Kinase to Mitotic Spindles.
Dev Cell. 2005 Aug;9(2):237-48. PDF
Pelletier L, Ozlu N, Hannak E, Cowan C, Habermann B, Ruer M, Muller-Reichert T, Hyman AA. The Caenorhabditis elegans centrosomal protein SPD-2 is required for both pericentriolar material recruitment and centriole duplication.
Curr Biol. 2004 May 25;14(10):863-73. PDF
Desai A, Rybina S, Muller-Reichert T, Shevchenko A, Shevchenko A, Hyman AA, Oegema K. KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans.
Genes Dev. 2003 Oct 1; 17(19):2421-35 PDF
Kirkham M, Mueller-Reichert T, Oegema K, Grill S, Hyman AA. SAS-4 is a C. elegans Centriolar Protein that Controls Centrosome Size.
Cell. 2003 Feb 21; 112 (575-587) PDF
Hannak E, Oegema K, Kirkham M, Gonczy P, Habermann B, Hyman AA. The kinetically dominant assembly pathway for centrosomal asters in Caenorhabditis elegans is gamma-tubulin dependent.
J Cell Biol. 2002 May 13; 157 (4): 591-602 PDF
Hannak E, Kirkham M, Hyman AA and Oegema K. Aurora-A kinase is required for centrosome maturation in C. elegans.
J Cell Biol. 2001 155 (7) 1109-1115 PDF
Wittmann T, Hyman AA, Desai A. The spindle: a dynamic assembly of microtubules and motors.
Nat Cell Biol. 2001 E28-34 PDF
Oegema K, Desai A, Rybina S, Kirkham M, Hyman AA. Functional analysis of kinetochore assembly in Caenorhabditis elegans.
J Cell Biol. 2001 153(6):1209-26 PDF

Polarity / Cortex

Brangwynne CP, Eckmann CR, Courson DS, Rybarska A, Hoege C, Gharakhani J, Jülicher F, Hyman AA. Germline P granules are liquid droplets that localize by controlled dissolution/condensation.
Science. 2009 Jun 26;324(5935):1729-32.
Schonegg S, Constantinescu AT, Hoege C, Hyman AA. The Rho GTPase-activating proteins RGA-3 and RGA-4 are required to set the initial size of PAR domains in Caenorhabditis elegans one-cell embryos.
Proc Natl Acad Sci U S A. 2007 Sep 18;104(38):14976-81. PDF
Zumdieck A, Kruse K, Bringmann H, Hyman AA, Jülicher F. Stress generation and filament turnover during actin ring constriction.
PLoS One. 2007 Aug 8;2(1):e696.
Bringmann H, Cowan CR, Kong J, Hyman AA. LET-99, GOA-1/GPA-16, and GPR-1/2 are required for aster-positioned cytokinesis.
Curr Biol. 2007 Jan 23;17(2):185-91. PDF
Pecreaux J, Röper JC, Kruse K, Jülicher F, Hyman AA, Grill SW, Howard J. Spindle oscillations during asymmetric cell division require a threshold number of active cortical force generators.
Curr Biol. 2006 Nov 7;16(21):2111-22.
Cowan CR, Hyman AA. Cyclin E-Cdk2 temporally regulates centrosome assembly and establishment of polarity in Caenorhabditis elegans embryos.
Nat Cell Biol. 2006 Dec;8(12):1441-7. PDF
Schonegg S and Hyman AA. CDC-42 and RHO-1 coordinate acto-myosin contractility and PAR protein localization during polarity establishment in C. elegans embryos.
Development 2006 Sep;133(18):3507-16. PDF
Bringmann H and Hyman AA. A cytokinesis furrow is positioned by two consecutive signals.
Nature 436, 731-734 (4 August 2005) PDF
Grill SW, Hyman AA. Spindle positioning by cortical pulling forces.
Dev Cell. 2005 Apr;8(4):461-5. PDF
Cowan CR, Hyman AA. Asymmetric cell division in C. elegans: cortical polarity and spindle positioning.
Annu Rev Cell Dev Biol. 2004;20:427-53. PDF
Cowan CR, Hyman AA. Centrosomes direct cell polarity independently of microtubule assembly in C. elegans embryos.
Nature 2004 Sep 2;431(7004):92-6. PDF
Grill SW, Howard J, Schaffer E, Stelzer EH, Hyman AA. The distribution of active force generators controls mitotic spindle position.
Science. 2003 Jul 25; 301 (5632):518-21. PDF
Grill, S. Gonczy P. Stelzer, E and Hyman AA.A. Polarity controls forces governing asymmetric spindle positioning in the Caenorhabditis elegans embryo.
Nature 2001 409(6820):630-3 PDF
Jantsch-Plunger V, Gonczy P, Romano A, Schnabel H, Hamill D, Schnabel R, Hyman AA, Glotzer M. CYK-4: A Rho Family GTPase Activating Protein (GAP) Required for Central Spindle Formation and Cytokinesis.
J. Cell Biol. 2000 149(7): 1391-1404 PDF

Genomics

Nina C. Hubner, Alexander W. Bird, Jürgen Cox, Bianca Splettstoesser, Peter Bandilla, Ina Poser, Anthony Hyman and Matthias Mann Quantitative proteomics combined with BAC TransgeneOmics reveals in vivo protein interactions
J Cell Biol. 2010 May 17;189(4):739-54.
Zhong M, Niu W, Lu ZJ, Sarov M, Murray JI, Janette J, Raha D, Sheaffer KL, Lam HY, Preston E, Slightham C, Hillier LW, Brock T, Agarwal A, Auerbach R, Hyman AA, Gerstein M, Mango SE, Kim SK, Waterston RH, Reinke V, Snyder M. Genome-wide identification of binding sites defines distinct functions for Caenorhabditis elegans PHA-4/FOXA in development and environmental response.
PLoS Genet. 2010 Feb 19;6(2):e1000848.
Boxem M, Maliga Z, Klitgord N, Li N, Lemmens I, Mana M, de Lichtervelde L, Mul JD, van de Peut D, Devos M, Simonis N, Yildirim MA, Cokol M, Kao HL, de Smet AS, Wang H, Schlaitz AL, Hao T, Milstein S, Fan C, Tipsword M, Drew K, Galli M, Rhrissorrakrai K, Drechsel D, Koller D, Roth FP, Iakoucheva LM, Dunker AK, Bonneau R, Gunsalus KC, Hill DE, Piano F, Tavernier J, van den Heuvel S, Hyman AA, Vidal M. A protein domain-based interactome network for C. elegans early embryogenesis.
Cell. 2008 Aug 8;134(3):534-45. PDF
Poser I, Sarov M, Hutchins JR, Hériché JK, Toyoda Y, Pozniakovsky A, Weigl D, Nitzsche A, Hegemann B, Bird AW, Pelletier L, Kittler R, Hua S, Naumann R, Augsburg M, Sykora MM, Hofemeister H, Zhang Y, Nasmyth K, White KP, Dietzel S, Mechtler K, Durbin R, Stewart AF, Peters JM, Buchholz F, Hyman AA. BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals.
Nat Methods. 2008 May;5(5):409-15 PDF
Kittler R, Pelletier L, Heninger AK, Slabicki M, Theis M, Miroslaw L, Poser I, Lawo S, Grabner H, Kozak K, Wagner J, Surendranath V, Richter C, Bowen W, Jackson AL, Habermann B, Hyman AA, Buchholz F. Genome-scale RNAi profiling of cell division in human tissue culture cells.
Nat Cell Biol. 2007 Dec;9(12):1401-12.
Sarov M, Schneider S, Pozniakovski A, Roguev A, Ernst S, Zhang Y, Hyman AA, Stewart AF. A recombineering pipeline for functional genomics applied to Caenorhabditis elegans.
Nat Methods. 2006 Oct;3(10):839-44. PDF
Kittler R, Pelletier L, Ma C, Poser I, Fischer S, Hyman AA, Buchholz F. RNA interference rescue by bacterial artificial chromosome transgenesis in mammalian tissue culture cells.
Proc Natl Acad Sci U S A. 2005 Feb 15;102(7):2396-401 PDF
Gunsalus KC, Ge H, Schetter AJ, Goldberg DS, Han JD, Hao T, Berriz GF, Bertin N, Huang J, Chuang LS, Li N, Mani R, Hyman AA, Sonnichsen B, Echeverri CJ, Roth FP, Vidal M, Piano F. Predictive models of molecular machines involved in Caenorhabditis elegans early embryogenesis.
Nature 2005 Aug 11;436(7052):861-5. PDF
Sonnichsen B, Koski LB, Walsh A, Marschall P, Neumann B, Brehm M, Alleaume AM, Artelt J, Bettencourt P, Cassin E, Hewitson M, Holz C, Khan M, Lazik S, Martin C, Nitzsche B, Ruer M, Stamford J, Winzi M, Heinkel R, Roder M, Finell J, Hantsch H, Jones SJ, Jones M, Piano F, Gunsalus KC, Oegema K, Gonczy P, Coulson A, Hyman AA, Echeverri CJ. Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans.
Nature 2005 Mar 24;434(7032):462-9 PDF
Gonczy P, Echeverri G, Oegema K, Coulson A, Jones SJ, Copley RR, Duperon J, Oegema J, Brehm M, Cassin E, Hannak E, Kirkham M, Pichler S, Flohrs K, Goessen A, Leidel S, Alleaume AM, Martin C, Ozlu N, Bork P, Hyman AA. Functional genomic analysis of cell division in C.elegans using RNAi of genes on chromosome III.
Nature 2000 408(6810): 331-6 PDF

Others

Petrásek Z, Hoege C, Mashaghi A, Ohrt T, Hyman AA, Schwille P. Characterization of protein dynamics in asymmetric cell division by scanning fluorescence correlation spectroscopy.
Biophys J. 2008 Dec;95(11):5476-86. PDF
Pozniakovsky AI, Knorre DA, Markova OV, Hyman AA, Skulachev VP, Severin FF. Role of mitochondria in the pheromone- and amiodarone-induced programmed death of yeast.
J Cell Biol. 2005 Jan 17;168(2):257-69 PDF
Hyman AA. Boveri revisited.
EMBO J. 2005 Mar 23;24(6):1104-10 PDF
Kurz T, Ozlu N, Rudolf F, O'Rourke SM, Luke B, Hofmann K, Hyman AA, Bowerman B, PeterM. The conserved protein DCN-1/Dcn1p is required for cullin neddylation in C. elegans and S. cerevisiae.
Nature 2005 Jun 30;435(7046):1257-61. PDF
Hyman AA, Howard J. Cell structure and dynamics.
Curr Opin Cell Biol. 2005 Feb;17(1):1-2. PDF