Selected Publications

Recent Publications

Recent Publications

 

Molecular biology: A liquid reservoir for silent chromatin.
Klosin A, Hyman AA. Nature (2017). [PDF]

 

The centrosome is a selective condensate that nucleates microtubules by concentrating tubulin.
Woodruff JB, Gomes BF, Widlund PO, Mahamid J, Honigmann A, Hyman AA. 
Cell. 2017. 169(6)1066-1077. [FullText]

 

Stem cells: the new “model organism.”
Drubin DG, Hyman AA.
Mol Biol Cell, 2017. 28(11)1409-1411. [FullText]

 

ATP as a biological hydrotrope. 
Patel A, Malinovska L, Saha S, Wang J, Alberti S, Krishnan Y, Hyman AA.
Science. 2017 May 18;356(6339):753-756.  [FullText][PDF][Video Abstract]

 

An aberrant phase transition of stress granules triggered by misfolded protein and prevented by chaperone function.
Mateju D, Franzmann TM, Patel A, Kopach A, Boczeck EE, Maharana S, Lee HO, Carra S, Hyman AA, Alberti S.
The EMBO Journal (2017) e201695957. [FullText]

 

Biomolecular condensates: organizers of cellular biochemistry.
Banani SF, Lee HO, Hyman AA, Rosen MK.
Nat Rev Mol Cell Biol. 2017 Feb 22. [PDF][PubMed]

 

Common molecular pathways in Amyotrophic Lateral Sclerosis and Frontotemporal Dementia.
Jochen H. Weishaupt, Tony Hyman, Ivan Dikic.
Trends in Molecular Medicine. 2016 Sep; 22(9):769-83. [PDF][PubMed]

 

Growth and division of active droplets provides a model for protocells
David Zwicker, Rabea Seyboldt, Christoph A. Weber, Anthony A. Hyman & Frank Jülicher
Nature Physics. 2016. [PDF][FullText]

 

The Mitotic Spindle in the One-Cell C. elegans Embryo Is Positioned with High Precision and Stability.
Pécréaux J, Redemann S, Alayan Z, Mercat B, Pastezeur S, Garzon-Coral C, Hyman AA, Howard J.
Biophys J. 2016 Oct 18;111(8):1773-1784. [FullText]

 

In vitro reconstitution of a membrane switch mechanism for the polarity protein LGL.
Visco I, Hoege C, Hyman AA, Schwille P.
J Mol Biol. 2016 Oct 6. pii: S0022-2836(16)30418-1. [PubMed]

 

Are aberrant phase transitions a driver of cellular aging?
Alberti S, Hyman AA.
Bioessays. 2016 Oct;38(10):959-68. doi: 10.1002/bies.201600042. Epub 2016 Aug 24. [FullText]

 

Polar Positioning of Phase-Separated Liquid Compartments in Cells Regulated by an mRNA Competition Mechanism.
Saha S, Weber CA, Nousch M, Adame-Arana O, Hoege C, Hein MY, Osborne-Nishimura E, Mahamid J, Jahnel M, Jawerth L, Pozniakovski A, Eckmann CR, Jülicher F, Hyman AA.
Cell. 2016 Sep 8;166(6):1572-1584.e16. doi: 10.1016/j.cell.2016.08.006. Epub 2016 Sep 1. [PubMed][VideoAbstract]

 

Polo-like kinase phosphorylation determines C. elegans centrosome size and density by biasing SPD-5 toward an assembly-competent conformation.
Wueseke O, Zwicker D, Schwager A, Wong YL, Ogema K, Jülicher F, Hyman AA, Woodruff JB. Biology Open 2016 5:1431-1440. [FullText][VideoAbstract]

 

Rheology of the Active Cell Cortex in Mitosis.
Fischer-Friedrich E, Toyoda Y, Cattin CJ, Müller DJ, Hyman AA, Jülicher F.
Biophys J. 2016; 111(3):589-600. [FullText]

 

Amyloid-like self-assembly of a cellular compartment.
Boke E, Ruer M, Wühr M, Coughlin M, Lemaitre R, Gygi SP, Alberti S, Drechsel D, Hyman AA, Mitchison T.
Cell. 2016; 166, 637-650. [PDF]

 

A locus in Pristionchus pacificus that is responsible for the ability to give rise to fertile offspring at higher temperatures.
Leaver M, Kienle S, Begasse ML, Sommer RJ, Hyman AA.
Biol Open. 2016 Jul 18. pii: bio.018127. doi: 10.1242/bio.018127. [FullText] [Video Abstract]

 

Priority of discovery in the life sciences.
Vale RD and Hyman AA.
eLife. 2016;5:e16931. [PDF]

 

Visualizing the molecular sociology at the HeLa cell nuclear periphery.
Mahamid J, Pfeffer S, Schaffer M, Villa E, Danev R, Cuellar LK, Förster F, Hyman AA, Plitzko JM, Baumeister W.
Science. 2016 Feb; 351(6276):969-72. [PubMed]

 

A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M.
Cell. 2015 Oct 22;163(3):712-23. doi: 10.1016/j.cell.2015.09.053. Epub 2015 Oct 22. [FullText][PubMed]

 

A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation
Patel A, Lee HO, Jawerth L, Maharana S, Jahnel M, Hein MY, Stoynov S, Mahamid J, Saha S, Franzmann TM, Pozniakovski A, Poser I, Maghelli N, Royer LA, Weigert M, Myers EW, Grill S, Drechsel D, Hyman AA, Alberti S.
Cell, 27 August 2015. [FullText] [Video Abstract]

 

Method: In vitro analysis of pericentriolar material assembly.
Woodruff JB, Hyman AA.
Methods Cell Biol. 2015;129:369-82. [PubMed] [PDF]

 

Centrosomes. Regulated assembly of a supramolecular scaffold in vitro.
Woodruff JB, Wueseke O, Viscardi V, Mahamid J, Ochoa SD, Bunkenborg J, Widlund PO, Pozniakovsky A, Zanin E, Bahmanyar S,Zinke A, Hong SH, Decker M, Baumeister W, Andersen JS, Oegema K, Hyman AA.
Science. 2015 May 15;348(6236):808-12. [FullText] [PDF Reprint]

 

Emergent properties of the metaphase spindle.
Reber S, Hyman AA.
Cold Spring Harb Perspect Biol. 2015 Jul 1;7(7). [PubMed] [PDF]

 

Temperature dependence of cell division timing accounts for a shift in the thermal limits of C. elegans and C. briggsae.
Begasse M, Leaver M, Vazquez F, Grill SW, Hyman AA.
Cell Rep. 2015 Feb 4; 10: 647-653. [FullText]

 

Liquid-liquid phase separation in biology.
Hyman AA, Weber CA, Jülicher F.
Annu Rev Cell Dev Biol. 2014 Oct 11;30:39-58. [PDF]

 

Quantification of surface tension and internal pressure generated by single mitotic cells.
Fischer-Friedrich, E, Hyman AA, Jülicher F, Müller DJ, Heleniusa J.
Sci Rep. 2014; 4: 6213. [Full Text]

 

The C. elegans pericentriolar material components SPD-2 and SPD-5 are monomeric in the cytoplasm prior to incorporation into the PCM matrix.
Wuekese O, Bunkenborg J, Hein MY, Zinke A, Viscardi V, Woodruff JB, Oegema K, Mann M, Andersen JS, Hyman AA.
Mol Biol Cell. 2014 Aug 7. pii: mbc.E13-09-0514. [PubMed]

 

Products of the Parkinson’s disease-related glyoxalase DJ-1, D-lactate and glycolate, support mitochondrial membrane potential and neuronal survival.
Toyoda Y, Erkut C, Pan-Montojo F, Boland S, Stewart MP, Müller DJ, Wurst W, Hyman AA, Kurzchalia T.
Biol Open. 2014 Jul 25. pii: BIO20149399. [FullText]

 

Pericentriolar material structure and dynamics.
Woodruff JB, Wueseke O, Hyman AA.
Phil. Trans. R. Soc. B. 2014 July; 369, 20130459. [PDF]

 

Encouraging Innovation.
Hyman AA.
Mol Biol Cell. 2014 Feb;25(4):427-8. [PDF]

Essays/Interviews

Essays/Interviews

 

Stem cells: the new “model organism.”
Mol Biol Cell, 2017. 28(11)1409-1411. [FullText]
Drubin DG, Hyman AA.

 

On Research Funding and the Power of Youth
ASCB Newsletter. 2016 Oct. [PDF]
Hyman AA, Desai A, Walter P.

 

Interview with Tony Hyman
Lab Times. 2016 Apr, p39. [FullText]

 

Priority of discovery in the life sciences.
eLife. 2016;5:e16931. [PDF]
Vale RD and Hyman AA.

 

Encouraging Innovation.
Mol Biol Cell. 2014 Feb;25(4):427-8. [PDF]
Hyman AA.

 

Funding Innovative Science.
Science. 2013 Jan. 11; Vol. 339 no. 6116 p. 119. [Article]
Hyman AA.

 

In Retrospect: The Origin of Life.
Nature. 2012 [PDF]
Hyman AA, Brangwynne CP

 

Some thoughts on German success.
DNN. 2012. [PDF] [online version]
Hyman AA.

 

ASCB Profile
ASCB Newsletter. 2012 Nov. [PDF]

 

How To Write a Paper
Tony Hyman [PDF]

 

Beyond Oil and Water — Phase Transitions in Cells
Science. 2012 Aug 31; 337: 1047. [PDF]
Hyman AA, Simons K

 

The new cell biology: Beyond HeLa cells
Nature. 2011 Nov 30;480(7375):34. [PDF][PubMed]
Hyman AA, Simons K

 

Whither systems biology
Phil. Trans. R. Soc. B. 2011 366, 3635-3637. [PDF]
Hyman AA.

 

Beyond Stereospecificity: Liquids and Mesoscale Organization of Cytoplasm
Dev Cell. 2011 Jul 19;21(1):14-6. [PDF]
Hyman AA, Brangwynne CP

 

Q & A – Tony Hyman
Curr Biol. 2011 Apr 12;21(7):R240-2. [PDF]

 

Automating Cell Biology: Genomics as an Industrial Revolution
ASCB Newsletter. 2011 Mar. [PDF]
Durbin R, Hyman AA

 

European vs. U.S. Careers: Your PhD as Your Passports
ASCB Newsletter. 2010 Oct. [PDF]
Hyman AA.

 

Anthony Hyman: From unlikely scientist to Royal Society Fellow
J Cell Biol. 2007 Dec 31;179(7):1330-1. [PDF]

 

A pipeline for Europe
Nature. 2007 Sep 13;449(7159):256. [PDF]
Hyman AA, Simons K

 

Boveri revisited (EMBO Gold Medal Review 2003)
EMBO J. 2005 Mar 23;24(6):1104-10. [PDF]
Hyman AA.

 

No man is an island…
Current Bio. 1996; 6(5):491. [PDF]
Hyman AA.

Reviews


Reviews

 

Klosin A, Hyman AA.
Molecular biology: A liquid reservoir for silent chromatin.
Nature2017 Jun 21. [PDF]

 

Banani SF, Lee HO, Hyman AA, Rosen MK.
Biomolecular condensates: organizers of cellular biochemistry.
Nat Rev Mol Cell Biol. 2017 Feb 22. [PDF]

 

Alberti S, Hyman AA.
Are aberrant phase transitions a driver of cellular aging?
Bioessays. 2016 Oct;38(10):959-68. doi: 10.1002/bies.201600042. Epub 2016 Aug 24. [FullText]

 

Hasse S, Hyman AA, Sarov M. TransgeneOmics – A transgenic platform for protein localization based function exploration.
Methods. 2015 Oct 17. pii: S1046-2023(15)30124-9. doi: 10.1016/j.ymeth.2015.10.005. [Epub ahead of print] Review. [PubMed]

 

Reber S, Hyman AA. Emergent properties of the metaphase spindle.
Cold Spring Harb Perspect Biol. 2015 Jul 1;7(7). [PubMed] [PDF]

 

Hyman AA, Weber CA, Jülicher F. Liquid-liquid phase separation in biology.
Annu Rev Cell Dev Biol. 2014 Oct 11;30:39-58. [PDF]

 

Woodruff JB, Wueseke O, Hyman AA. Pericentriolar material structure and dynamics.
Phil. Trans. R. Soc. B. 2014 July; 369, 20130459. [PDF]

 

Hoege C, Hyman AA.  Principles of PAR polarity in Caenorhabditis elegans embryos.
Nat Rev Mol Cell Biol. 2013 May;14(5):315-22.  [PubMed] [PDF]

 

Goehring NW, Hyman AA.  Organelle Growth Control through Limiting Pools of Cytoplasmic Components.
Curr Biol. 2012 May 8;22(9):R330-9.  [PubMed]

 

Reber S, Hyman AA. Samurai sword sets spindle size.
Cell. 2011 Dec 9;147(6):1224-5. [PubMed]

 

Howard J, Hyman AA. Growth, fluctuation and switching at microtubule plus ends.
Nat Rev Mol Cell Biol. 2009 Jun 10. [PDF]

 

Cowan CR, Hyman AA.  Acto-myosin reorganization and PAR polarity in C. elegans.
Development. 2007 Mar;134(6):1035-43. [PDF]

 

Howard J, Hyman AA.  Microtubule polymerases and depolymerases.
Curr Opin Cell Biol. 2007 Feb;19(1):31-5. [PDF]

 

Oegema K, Hyman AA. Cell division.
WormBook. 2006 Jan 19:1-40.[WormBook]

 

Kinoshita K, Noetzel TL, Arnal I, Drechsel DN, Hyman AA.  Global and local control of microtubule destabilization promoted by a catastrophe kinesin MCAK/XKCM1.
J Muscle Res Cell Motil.  2006;27(2):107-14. [PDF]

 

Hyman AA, Howard J. Cell structure and dynamics. 
Curr Opin Cell Biol. 2005 Feb;17(1):1-2. [PDF]

 

Cowan CR, Hyman AA.   Asymmetric cell division in C. elegans: cortical polarity and spindle positioning.
Annu Rev Cell Dev Biol. 2004;20:427-53. [PDF]

 

Howard J and Hyman AA. Dynamics and mechanics of the microtubule plus end.
Nature. 2003; 422 (6933): 753-8. [PDF]

 

Wittmann T, Hyman AA, Desai A.  The spindle: a dynamic assembly of microtubules and motors.
Nat Cell Biol. 2001 E28-34.[PDF]

 

Hyman AA. Centrosomes: Sic transit gloria centri
Curr Biol. 2000 Apr 6;10(7):R276-8.  [Article]

Liquids

Liquids

 

Patel A, Malinovska L, Saha S, Wang J, Alberti S, Krishnan Y, Hyman AA.
ATP as a biological hydrotrope. 
Science. 2017 May 18;356(6339):753-756.  [FullText][PDF][Video Abstract]

 

Mateju D, Franzmann TM, Patel A, Kopach A, Boczeck EE, Maharana S, Lee HO, Carra S, Hyman AA, Alberti S.
An aberrant phase transition of stress granules triggered by misfolded protein and prevented by chaperone function.
The EMBO Journal. (2017) e201695957. [FullText]

 

David Zwicker, Rabea Seyboldt, Christoph A. Weber, Anthony A. Hyman & Frank Jülicher
Growth and division of active droplets provides a model for protocells
Nature Physics. 2016. [PDF][FullText]

 

Weishaupt JH, Hyman AA, Dikic I.
Common molecular pathways in Amyotrophic Lateral Sclerosis and Frontotemporal Dementia.
Trends in Molecular Medicine. 2016 Sep; 22(9):769-83. [PDF][PubMed]

 

Saha S, Weber CA, Nousch M, Adame-Arana O, Hoege C, Hein MY, Osborne-Nishimura E, Mahamid J, Jahnel M, Jawerth L, Pozniakovski A, Eckmann CR, Jülicher F, Hyman AA.
Polar Positioning of Phase-Separated Liquid Compartments in Cells Regulated by an mRNA Competition Mechanism.
Cell. 2016 Sep 8;166(6):1572-1584.e16. doi: 10.1016/j.cell.2016.08.006. Epub 2016 Sep 1. [PubMed][VideoAbstract]

 

Boke E, Ruer M, Wühr M, Coughlin M, Lemaitre R, Gygi SP, Alberti S, Drechsel D, Hyman AA, Mitchison T. Amyloid-like self-assembly of a cellular compartment.
Cell. 2016; 166, 637-650. [PDF]

 

Patel A, Lee HO, Jawerth L, Maharana S, Jahnel M, Hein MY, Stoynov S, Mahamid J, Saha S, Franzmann TM, Pozniakovski A, Poser I, Maghelli N, Royer LA, Weigert M, Myers EW, Grill S, Drechsel D, Hyman AA, Alberti S. A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation.
Cell, 27 August 2015. [FullText][VideoAbstract]

 

Zwicker D, Hyman AA, Jülicher F. Suppression of Ostwald ripening in active emulsions.
Phys Rev E. 2015 Jul 1; 92(1):012317. [Abstract]

 

Hyman AA, Weber CA, Jülicher F. Liquid-liquid phase separation in biology.
Annu Rev Cell Dev Biol. 2014 Oct 11;30:39-58. [PDF]

 

Lee CF, Brangwynne CP, Gharakhani J, Hyman AA, Jülicher F. Spatial organization of the cell cytoplasm by position-dependent phase separation.
Phys Rev Lett. 2013 Aug 23; 111(8):088101. [PubMed][PDF]

 

Hyman AA, Simons K.  Cell Biology. Beyond oil and water–phase transitions in cells.
Science. 2012 Aug 31;337(6098):1047-1049. [PubMed][PDF]

 

Hyman AA, Brangwynne, CP.  Beyond Stereospecificity: Liquids and Mesoscale Organization of Cytoplasm
Dev Cell. 2011 Jul 19;21(1):14-6. [PDF]

 

Brangwynne CP, Mitchison TJ, Hyman AA. Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes.
Proc Natl Acad Sci U S A. 2011 Mar 15;108(11):4334-9. (Epub 2011 Feb 28) [PubMed]

 

Brangwynne CP, Eckmann CR, Courson DS, Rybarska A, Hoege C, Gharakhani J, Jülicher F, Hyman AA.  Germline P granules are liquid droplets that localize by controlled dissolution/condensation.
Science. 2009 Jun 26;324(5935):1729-32.  [PubMed]

Cell Rounding

Cell Rounding

 

Fischer-Friedrich E, Toyoda Y, Cattin CJ, Müller DJ, Hyman AA, Jülicher F. Rheology of the Active Cell Cortex in Mitosis.
Biophys J. 2016; 111(3):589-600. [FullText]

 

Sorce B, Escobedo C, Toyoda Y, Stewart MP, Cattin CJ, Newton R, Banerjee I, Stettler A, Roska B, Eaton S, Hyman AA, Hierlemann A, Müller DJ. Mitotic cells contract actomyosin cortex and generate pressure to round against or escape epithelial confinement.
Nat Commun. 2015 Nov;6:88872. [PubMed]

 

Fischer-Friedrich, E, Hyman AA, Jülicher F, Müller DJ, Heleniusa J. Quantification of surface tension and internal pressure generated by single mitotic cells.
Sci Rep. 2014; 4: 6213. [Full Text]

 

Stewart MP, Toyoda Y, Hyman AA, Müller DJTracking mechanics and volume of globular cells with atomic force microscopy using a constant-height clamp.
Nat Protoc. 2012 Jan;7(1):143-54.  [PubMed]

 

Toyoda Y, Stewart MP, Hyman AA, Müller DJ.  Atomic Force Microscopy to Study Mechanics of Living Mitotic Mammalian Cells.
Jpn. J. Appl. Phys.  2011: 50 08LA01.  [Journal Abstract]

 

Stewart MP, Toyoda Y, Hyman AA, Muller DJ. Force probing cell shape changes to molecular resolution.
Trends Biochem Sci. 2011 Aug;36(8):444-50. (Epub 2011 Jun 7)  [PubMed]

 

Stewart MP, Helenius J, Toyoda Y, Ramanathan SP, Muller DJ, Hyman AA. Hydrostatic pressure and the actomyosin cortex drive mitotic cell rounding.
Nature. 2011 Jan 13;469(7329):226-30. [PubMed]

Centrosomes & Spindles

Centrosomes & Spindles

 

Woodruff JB, Gomes BF, Widlund PO, Mahamid J, Honigmann A, Hyman AA. 
The centrosome is a selective condensate that nucleates microtubules by concentrating tubulin.
Cell. 2017. 169(6)1066-1077. [FullText]

 

Pécréaux J, Redemann S, Alayan Z, Mercat B, Pastezeur S, Garzon-Coral C, Hyman AA, Howard J.
The Mitotic Spindle in the One-Cell C. elegans Embryo Is Positioned with High Precision and Stability.
Biophys J. 2016 Oct 18;111(8):1773-1784. [FullText]

 

Wueseke O, Zwicker D, Schwager A, Wong YL, Ogema K, Jülicher F, Hyman AA, Woodruff JB. Polo-like kinase phosphorylation determines C. elegans centrosome size and density by biasing SPD-5 toward an assembly-competent conformation.
Biology Open 2016 5:1431-1440. [FullText][VideoAbstract]

 

Woodruff JB, Hyman AA. Method: In vitro analysis of pericentriolar material assembly.
Methods Cell Biol. 2015;129:369-82. [PubMed] [PDF]

 

Reber S, Hyman AA. Emergent properties of the metaphase spindle.
Cold Spring Harb Perspect Biol. 2015 Jul 1;7(7). [PubMed] [PDF]

 

Woodruff JB, Wueseke O, Viscardi V, Mahamid J, Ochoa SD, Bunkenborg J, Widlund PO, Pozniakovsky A, Zanin E, Bahmanyar S,Zinke A, Hong SH, Decker M, Baumeister W, Andersen JS, Oegema K, Hyman AA. Centrosomes. Regulated assembly of a supramolecular scaffold in vitro.
Science. 2015 May 15;348(6236):808-12. [FullText] [PDF Reprint]

 

Wuekese O, Bunkenborg J, Hein MY, Zinke A, Viscardi V, Woodruff JB, Oegema K, Mann M, Andersen JS, Hyman AA. The C. elegans pericentriolar material components SPD-2 and SPD-5 are monomeric in the cytoplasm prior to incorporation into the PCM matrix.
Mol Biol Cell. 2014 Aug 7. pii: mbc.E13-09-0514. [PubMed]

 

Woodruff JB, Wueseke O, Hyman AA. Pericentriolar material structure and dynamics.
Phil. Trans. R. Soc. B. 2014 July; 369, 20130459. [PDF]

 

Zwicker D, Decker M, Jaensch S, Hyman AA, Jülicher F. Centrosomes are autocatalytic droplets of pericentriolar material organized by centrioles.
PNAS. 2014 Jul 1;111(26):E2636-45. [PubMed]

 

Kuhn M, Hyman AA, Beyer A. Coiled-coil proteins facilitated the functional expansion of the centrosome.
PLoS Comput Biol. 2014 Jun 5;10(6)e1003657. [PubMed]

 

Reber SB, Baumgart J, Widlund PO, Pozniakovsky A, Howard J, Hyman AA, Jülicher F. XMAP215 activity sets spindle length by controlling the total mass of spindle microtubules.
Nat Cell Biol. 2013 Aug 25. doi: 10.1038/ncb2834. [Epub ahead of print] [PDF] [PubMed]

 

Decker M, Jaensch S, Pozniakovsky A, Zinke A, O’Connell KF, Zachariae W, Myers E, Hyman AA.  Limiting Amounts of Centrosome Material Set Centrosome Size in C. elegans Embryos.
Curr Biol. 2011 Aug 9;21(15):1259-67. (Epub 2011 Jul 28)  [PDF]

 

Jakobsen L, Vanselow K, Skogs M, Toyoda Y, Lundberg E, Poser I, Falkenby LG, Bennetzen M, Westendorf J, Nigg EA, Uhlen M, Hyman AA, Andersen JS.  Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods.
EMBO J. 2011 Apr 20;30(8):1520-35. (Epub 2011 Mar 11)  [PDF]

 

Greenan G, Brangwynne CP, Jaensch S, Gharakhani J, Jülicher F, Hyman AA.  Centrosome size sets mitotic spindle length in Caenorhabditis elegans embryos.
Curr Biol. 2010 Feb 23;20(4):353-8. (Epub 2010 Feb 4) [PDF]

 

Jaensch S, Decker M, Hyman AA, Myers EW.  Automated tracking and analysis of centrosomes in early Caenorhabditis elegans embryos.
Bioinformatics. 2010 Jun 15;26(12):i13-20.  [PDF]

 

Lawo S, Bashkurov M, Mullin M, Ferreria MG, Kittler R, Habermann B, Tagliaferro A, Poser I, Hutchins JR, Hegemann B, Pinchev D, Buchholz F, Peters JM, Hyman AA, Gingras AC, Pelletier L.  HAUS, the 8-subunit human Augmin complex, regulates centrosome and spindle integrity.
Curr Biol. 2009 May 26;19(10):816-26. [PDF]

 

Boxem M, Maliga Z, Klitgord N, Li N, Lemmens I, Mana M, de Lichtervelde L, Mul JD, van de Peut D, Devos M, Simonis N, Yildirim MA, Cokol M, Kao HL, de Smet AS, Wang H, Schlaitz AL, Hao T, Milstein S, Fan C, Tipsword M, Drew K, Galli M, Rhrissorrakrai K, Drechsel D, Koller D, Roth FP, Iakoucheva LM, Dunker AK, Bonneau R, Gunsalus KC, Hill DE, Piano F, Tavernier J, van den Heuvel S, Hyman AA, Vidal M.  A protein domain-based interactome network for C. elegans early embryogenesis.
Cell. 2008 Aug 8;134(3):534-45.  [PDF]

 

Zhu F, Lawo S, Bird A, Pinchev D, Ralph A, Richter C, Müller-Reichert T, Kittler R, Hyman AA, Pelletier L.  The mammalian SPD-2 ortholog Cep192 regulates centrosome biogenesis.
Curr Biol. 2008 Jan 22;18(2):136-41.  [PDF]

 

Schlaitz AL, Srayko M, Dammermann A, Quintin S, Wielsch N, MacLeod I, de Robillard Q, Zinke A, Yates III JR, Müller-Reichert T, Shevchenko A, Oegema K, Hyman AA.   The C. elegans RSA Complex Localizes Protein Phosphatase 2A to Centrosomes and Regulates Mitotic Spindle Assembly.
Cell 2007, January 12; 128(1):115-127.  [Article]

 

Pelletier L, O’Toole E, Schwager A, Hyman AA, Müller-Reichert T.   Centriole assembly in Caenorhabditis elegans.
Nature. 2006 Nov 30;444(7119):619-23.  [PDF]

 

Kinoshita K, Noetzel TL, Pelletier L, Mechtler K, Drechsel DN, Schwager A, Lee M, Raff JW, Hyman AA.   Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis. 
J Cell Biol. 2005 Sep 26;170(7):1047-55. [PDF]

 

Ozlu N, Srayko M, Kinoshita K, Habermann B, O’toole ET, Muller-Reichert T, Schmalz N, Desai A, Hyman AA.   An Essential Function of the C. elegans Ortholog of TPX2 Is to Localize Activated Aurora A Kinase to Mitotic Spindles.
Dev Cell. 2005 Aug;9(2):237-48.  [PDF]

 

Pelletier L, Ozlu N, Hannak E, Cowan C, Habermann B, Ruer M, Muller-Reichert T, Hyman AA.   The Caenorhabditis elegans centrosomal protein SPD-2 is required for both pericentriolar material recruitment and centriole duplication.
Curr Biol. 2004 May 25;14(10):863-73.  [PDF]

 

Desai A, Rybina S, Muller-Reichert T, Shevchenko A, Shevchenko A, Hyman AA, Oegema K.  KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans.
Genes Dev. 2003 Oct 1; 17(19):2421-35.  [PDF]

 

Kirkham M, Mueller-Reichert T, Oegema K, Grill S, Hyman AA.  SAS-4 is a C. elegans Centriolar Protein that Controls Centrosome Size.
Cell. 2003 Feb 21; 112 (575-587).  [PDF]

 

Hannak E, Oegema K, Kirkham M, Gonczy P, Habermann B, Hyman AA.  The kinetically dominant assembly pathway for centrosomal asters in Caenorhabditis elegans is gamma-tubulin dependent.
J Cell Biol. 2002 May 13; 157 (4): 591-602.  [PDF]

 

Hannak E, Kirkham M, Hyman AA and Oegema K.   Aurora-A kinase is required for centrosome maturation in C. elegans.
J Cell Biol. 2001 155 (7) 1109-1115.  [PDF]

Microtubules

Microtubules

 

Reber SB, Baumgart J, Widlund PO, Pozniakovsky A, Howard J, Hyman AA, Jülicher F. XMAP215 activity sets spindle length by controlling the total mass of spindle microtubules.
Nat Cell Biol. 2013 Aug 25. doi: 10.1038/ncb2834. [Epub ahead of print] [PDF] [PubMed]

 

Zanic M, Widlund PO, Hyman AA, Howard J. Synergy between XMAP215 and EB1 increases microtubule growth rates to physiological levels.
Nat Cell Biol. 2013 Jun;15(6):688-93. [PubMed] [PDF]

 

Scolz M, Widlund PO, Piazza S, Bublik DR, Reber S, Peche LY, Ciani Y, Hubner N, Isokane M, Monte M, Ellenberg J, Hyman AA, Schneider C, Bird AW.  GTSE1 Is a Microtubule Plus-End Tracking Protein That Regulates EB1-Dependent Cell Migration.
PLoS One 2012 Dec. 20 [Epub ahead of print][PubMed]

 

Widlund PO, Podolski M, Reber S, Alper J, Storch M, Hyman AA, Howard J, and Drechsel DN.  One-step purification of assembly competent tubulin from diverse eukaryotic sources.
Mol Biol Cell. 2012 Nov;23(22):4393-401. (Epub 2012 Sep 19) [PubMed]

 

Weber B, Greenan G, Prohaska S, Baum D, Hege HC, Müller-Reichert T, Hyman AA, Verbavatz JM. Automated tracing of microtubules in electron tomograms of plastic embedded samples of Caenorhabditis elegans embryos.
J Struct Biol. 2012; 178(2): 129-138. [PubMed]

 

Widlund PO, Stear JH, Pozniakovsky A, Zanic M, Reber S, Brouhard GJ, Hyman AA, Howard J.  XMAP215 polymerase activity is built by combining multiple tubulin-binding TOG domains and a basic lattice-binding region.
Proc Natl Acad Sci U S A. 2011 Feb 15;108(7):2741-6. (Epub 2011 Jan 31) [PDF]

 

Zanic M, Stear JH, Hyman AA, Howard J. EB1 recognizes the nucleotide state of tubulin in the microtubule lattice.
PLoS One. 2009 Oct 23;4(10):e7585.[PDF]

 

Brouhard GJ, Stear JH, Noetzel TL, Al-Bassam J, Kinoshita K, Harrison SC, Howard J, Hyman AA. XMAP215 is a processive microtubule polymerase.
Cell 2008 Jan 11;132(1):79-88.[PDF]

 

Elie-Caille C, Severin F, Helenius J, Howard J, Muller DJ, Hyman AA.  Straight GDP-tubulin protofilaments form in the presence of taxol.
Curr Biol. 2007 Oct 23;17(20):1765-70.[PDF]

 

Al-Bassam J, Larsen NA, Hyman AA, Harrison SC. Crystal structure of a TOG domain: conserved features of XMAP215/Dis1-family TOG domains and implications for tubulin binding.
Structure. 2007 Mar;15(3):355-62.[PDF]

 

Al-Bassam J, van Breugel M, Harrison SC, Hyman AA. Stu2p binds tubulin and undergoes an open-to-closed conformational change.
J Cell Biol. 2006 Mar 27;172(7):1009-22.[PDF]

 

Barros TP, Kinoshita K, Hyman AA, Raff JW. Aurora A activates D-TACC-Msps complexes exclusively at centrosomes to stabilize centrosomal microtubules.
J Cell Biol. 2005 Sep 26;170(7):1039-46.[PDF]

 

Srayko M, Kaya A, Stamford J, Hyman AA. Identification and Characterization of Factors Required for Microtubule Growth and Nucleation in the Early C. elegans Embryo.
Dev Cell. 2005 Aug;9(2):223-36.[PDF]

 

Noetzel TL, Drechsel DN, Hyman AA, Kinoshita K. A comparison of the ability of XMAP215 and tau to inhibit the microtubule destabilizing activity of XKCM1.
Philos Trans R Soc Lond B Biol Sci. 2005 Mar 29;360(1455):591-4.[PDF]

 

Van Breugel M, Hyman AA, Drechsel D. Stu2p, the budding yeast member of the conserved Dis1/XMAP215 family of microtubule-associated proteins is a plus end-binding microtubule destabilizer.
J Cell Biol. 2003 April 28; 161 (2): 1-13.[PDF]

 

Srayko M, Quintin S, Schwager A, Hyman AA. Caenorhabditis elegans TAC-1 and ZYG-9 form a complex that is essential for long astral and spindle microtubules.
Curr Biol. 2003 Sep 2;13(17):1506-11.[PDF]

 

Kinoshita K, Arnal I, Desai A, Drechsel DN, Hyman AA. Reconstitution of Physiological Microtubule Dynamics Using Purified Components.
Science. 2001; 294: 1340-1343.[PDF]

 

Arnal I, Karsenti E, Hyman AA. Structural Transitions at microtubule ends correlate with their dynamic properties in Xenopus egg extracts.
J Cell Biol. 2000; 149: 767-774.[PDF]

Polarity/Cortex

Polarity/Cortex

 

Visco I, Hoege C, Hyman AA, Schwille P.
In vitro reconstitution of a membrane switch mechanism for the polarity protein LGL.
J Mol Biol. 2016 Oct 6. pii: S0022-2836(16)30418-1. [PubMed]

 

Saha S, Weber CA, Nousch M, Adame-Arana O, Hoege C, Hein MY, Osborne-Nishimura E, Mahamid J, Jahnel M, Jawerth L, Pozniakovski A, Eckmann CR, Jülicher F, Hyman AA.
Polar Positioning of Phase-Separated Liquid Compartments in Cells Regulated by an mRNA Competition Mechanism.
Cell. 2016 Sep 8;166(6):1572-1584.e16. doi: 10.1016/j.cell.2016.08.006. Epub 2016 Sep 1. [PubMed][VideoAbstract]

 

Goehring NW, Trong PK, Bois JS, Chowdhury D, Nicola EM, Hyman AA, Grill SW. Polarization of PAR proteins by advective triggering of a pattern-forming system
Science. 2011 Nov 25;334(6059):1137-41.  [PubMed]

 

Goehring NW, Hoege C, Grill SW, Hyman AA.  PAR proteins diffuse freely across the anterior-posterior boundary in polarized C. elegans embryos.
J Cell Biol. 2011 May 2;193(3):583-94. (Epub 2011 Apr 25)  [PubMed]

 

Stewart MP, Helenius J, Toyoda Y, Ramanathan SP, Muller DJ, Hyman AA.  Hydrostatic pressure and the actomyosin cortex drive mitotic cell rounding.
Nature. 2011 Jan 13;469(7329):226-30. (Epub 2011 Jan 2)  [Article]

 

Goehring NW, Chowdhury D, Hyman AA, Grill SW.  FRAP Analysis of Membrane-Associated Proteins: Lateral Diffusion and Membrane-Cytoplasmic Exchange.
Biophys J. 2010 Oct 20;99(8):2443-52.  [PubMed]

 

Redemann S, Pecreaux J, Goehring NW, Khairy K, Stelzer EH, Hyman AA, Howard J.  Membrane Invaginations Reveal Cortical Sites that Pull on Mitotic Spindles in One-Cell C. elegans Embryos.
PLoS One. 2010 Aug 20;5(8).  [Article]

 

Hoege C, Constantinescu AT, Schwager A, Goehring NW, Kumar P, Hyman AA. LGL can partition the cortex of one-cell Caenorhabditis elegans embryos into two domains.
Curr Biol. 2010 Jul 27;20(14):1296-303. (Epub 2010 Jun 24)  [PubMed]

 

Petrásek Z, Hoege C, Mashaghi A, Ohrt T, Hyman AA, Schwille P.   Characterization of protein dynamics in asymmetric cell division by scanning fluorescence correlation spectroscopy.
Biophys J. 2008 Dec;95(11):5476-86.  [PubMed]

 

Pecreaux J, Röper JC, Kruse K, Jülicher F, Hyman AA, Grill SW, Howard J.   Spindle oscillations during asymmetric cell division require a threshold number of active cortical force generators.
Curr Biol. 2006 Nov 7;16(21):2111-22.  [PubMed]

 

Cowan CR, Hyman AA.  Cyclin E-Cdk2 temporally regulates centrosome assembly and establishment of polarity in Caenorhabditis elegans embryos.
Nat Cell Biol. 2006 Dec;8(12):1441-7.  [PubMed]

 

Schonegg S and Hyman AA.  CDC-42 and RHO-1 coordinate acto-myosin contractility and PAR protein localization during polarity establishment in C. elegans embryos. 
Development 2006 Sep;133(18):3507-16.  [PubMed]

 

Bringmann H and Hyman AA. A cytokinesis furrow is positioned by two consecutive signals.
Nature 436, 731-734 (4 August 2005).  [Article]

 

Cowan CR, Hyman AA.  Centrosomes direct cell polarity independently of microtubule assembly in C. elegans embryos
Nature. 2004 Sep 2;431(7004):92-6.  [Article]

 

Grill SW, Howard J, Schaffer E, Stelzer EH, Hyman AA.  The distribution of active force generators controls mitotic spindle position.
Science 2003 Jul 25; 301 (5632):518-21.  [PubMed]

 

Grill, S. Gonczy P. Stelzer, E and Hyman AA.  Polarity controls forces governing asymmetric spindle positioning in the Caenorhabditis elegans embryo. 
Nature 2001; 409(6820):630-3.  [PubMed]

 

Jantsch-Plunger V, Gonczy P, Romano A, Schnabel H, Hamill D, Schnabel R, Hyman AA, Glotzer M.   CYK-4: A Rho Family GTPase Activating Protein (GAP) Required for Central Spindle Formation and Cytokinesis.
J. Cell Biol. 2000; 149(7): 1391-1404.  [PubMed]

Genomics

Genomics

 

Hasse S, Hyman AA, Sarov M. TransgeneOmics – A transgenic platform for protein localization based function exploration.
Methods. 2015 Oct 17. pii: S1046-2023(15)30124-9. doi: 10.1016/j.ymeth.2015.10.005. [Epub ahead of print] Review. [PubMed]

 

Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M. A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances.
Cell. 2015 Oct 22;163(3):712-23. doi: 10.1016/j.cell.2015.09.053. Epub 2015 Oct 22. [FullText][PubMed]

 

Maliga Z, Junqueira M, Toyoda Y, Ettinger A, Mora-Bermúdez F, Klemm RW, Vasilj A, Guhr E, Ibarlucea-Benitez I, Poser I, Bonifacio E, Huttner WB, Shevchenko A, Hyman AA. A genomic toolkit to investigate kinesin and myosin motor function in cells.
Nat Cell Biol. 2013 Mar;15(3):325-34. [PubMed] [PDF]

 

Bassik MC, Kampmann M, Lebbink RJ, Wang S, Hein MY, Poser I, Weibezahn J, Horlbeck MA, Chen S, Mann M, Hyman AA, Leproust EM, McManus MT, Weissman JS. A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility.
Cell. 2013 Feb 14;152(4):909-22. [PubMed] [PDF]

 

Sarov M, Murray JI, Schanze K, Pozniakovski A, Niu W, Angermann K, Hasse S, Rupprecht M, Vinis E, Tinney M, Preston E, Zinke A, Enst S, Teichgraber T, Janette J, Reis K, Janosch S, Schloissnig S, Ejsmont RK, Slightam C, Xu X, Kim SK, Reinke V, Stewart AF, Snyder M, Waterston RH, Hyman AA. A genome-scale resource for in vivo tag-based protein function exploration in C. elegans.
Cell. 2012 Aug 17;150(4):855-66. [PubMed] [PDF]

 

Neumann B, Walter T, Hériché JK, Bulkescher J, Erfle H, Conrad C, Rogers P, Poser I, Held M, Liebel U, Cetin C, Sieckmann F, Pau G, Kabbe R, Wünsche A, Satagopam V, Schmitz MH, Chapuis C, Gerlich DW, Schneider R, Eils R, Huber W, Peters JM, Hyman AA, Durbin R, Pepperkok R, Ellenberg J.   Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes.
Nature. 2010 Apr 1;464(7289):721-7. [PubMed]

 

Hutchins JR, Toyoda Y, Hegemann B, Poser I, Hériché JK, Sykora MM, Augsburg M, Hudecz O, Buschhorn BA, Bulkescher J, Conrad C, Comartin D, Schleiffer A, Sarov M, Pozniakovsky A, Slabicki MM, Schloissnig S, Steinmacher I, Leuschner M, Ssykor A, Lawo S, Pelletier L, Stark H, Nasmyth K, Ellenberg J, Durbin R, Buchholz F, Mechtler K, Hyman AA, Peters JM.   Systematic analysis of human protein complexes identifies chromosome segregation proteins.
Science. 2010 Apr 30;328(5978):593-9. (Epub 2010 Apr 1) [PubMed]

 

Kittler R, Pelletier L, Heninger AK, Slabicki M, Theis M, Miroslaw L, Poser I, Lawo S, Grabner H, Kozak K, Wagner J, Surendranath V, Richter C, Bowen W, Jackson AL, Habermann B, Hyman AA, Buchholz F.   Genome-scale RNAi profiling of cell division in human tissue culture cells.
Nat Cell Biol. 2007 Dec;9(12):1401-12.  [PubMed]

 

Sonnichsen B, Koski LB, Walsh A, Marschall P, Neumann B, Brehm M, Alleaume AM, Artelt J, Bettencourt P, Cassin E, Hewitson M, Holz C, Khan M, Lazik S, Martin C, Nitzsche B, Ruer M, Stamford J, Winzi M, Heinkel R, Roder M, Finell J, Hantsch H, Jones SJ, Jones M, Piano F, Gunsalus KC, Oegema K, Gonczy P, Coulson A, Hyman AA, Echeverri CJ.  Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans
Nature 2005 Mar 24;434(7032):462-9.  [PubMed]

 

Gonczy P, Echeverri G, Oegema K, Coulson A, Jones SJ, Copley RR, Duperon J, Oegema J, Brehm M, Cassin E, Hannak E, Kirkham M, Pichler S, Flohrs K, Goessen A, Leidel S, Alleaume AM, Martin C, Ozlu N, Bork P, Hyman AA.   Functional genomic analysis of cell division in C.elegans using RNAi of genes on chromosome III.
Nature 2000 408(6810): 331-6.  [PubMed]

BAC Pubs


BAC Engineering

 

Bird AW, Erler A, Fu J, Hériché JK, Maresca M, Zhang Y, Hyman AA, Stewart AF. High-efficiency counterselection recombineering for site-directed mutagenesis in bacterial artificial chromosomes
Nat Methods. 2011 Dec 4;9(1):103-9.  [PubMed]

 

Poser I, Sarov M, Hutchins JR, Hériché JK, Toyoda Y, Pozniakovsky A, Weigl D, Nitzsche A, Hegemann B, Bird AW, Pelletier L, Kittler R, Hua S, Naumann R, Augsburg M, Sykora MM, Hofemeister H, Zhang Y, Nasmyth K, White KP, Dietzel S, Mechtler K, Durbin R, Stewart AF, Peters JM, Buchholz F, Hyman AA. BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals. 
Nat Methods. 2008 May;5(5):409-15.  [PubMed]

 

Bird AW, Hyman AA.  Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A.
J Cell Biol. 2008 Jul 28;182(2):289-300. [PubMed]

 

Sarov M, Schneider S, Pozniakovski A, Roguev A, Ernst S, Zhang Y, Hyman AA, Stewart AF.  A recombineering pipeline for functional genomics applied to Caenorhabditis elegans.
Nat Methods. 2006 Oct;3(10):839-44.  [PubMed]

 

Kittler R, Pelletier L, Ma C, Poser I, Fischer S, Hyman AA, Buchholz F. RNA interference rescue by bacterial artificial chromosome transgenesis in mammalian tissue culture cells
Proc Natl Acad Sci U S A. 2005 Feb 15;102(7):2396-401.  [PubMed]

BAC Transformed Cell Lines

 

Maliga Z, Junqueira M, Toyoda Y, Ettinger A, Mora F, Klemm RW, Vasilj A, Guhr E, Benitez I, Poser I, Simons K, Bonifacio E, Huttner W, Shevchenko A, Hyman AA.   A genomic toolkit to investigate kinesin and myosin motor function in cells.
Nat Cell Biology

 

Uzunova K, Dye BT, Schutz H, Ladurner R, Petzold G, Toyoda Y, Jarvis MA, Brown NG, Poser I, Novatchkova M, Mechtler K, Hyman AA, Stark H, Schulman BA, Peters JM.   APC15 mediates CDC20 autoubiquitylation by APC/C(MCC) and disassembly of the mitotic checkpoint complex.
Nat Struct Mol Biol. 2012 Sep 24. [PubMed]

 

Splinter D, Razafsky DS, Schlager MA, Serra-Marques A, Grigoriev I, Demmers J, Keijzer N, Jiang K, Poser I, Hyman AA, Hoogenraad CC, King SJ, Akhmanova A.   BICD2, dynactin and LIS1 cooperate in regulating dynein recruitment to cellular structures.
Mol Biol Cell 2012 Sep 5. [PubMed]

 

Wurzenberger C, Held M, Lampson MA, Poser I, Hyman AA, Gerlich DW.   Sds22 and Repo-Man stabilize chromosome segregation by counteracting Aurora B on anaphase kinetochores.
J Cell Biol. 2012 Jul 16. [PubMed]

 

Frost A, Elgort MG, Brandman O, Ives C, Collins SR, Miller-Vedam L, Weibezahn J, Hein MY, Poser I, Mann M, Hyman AA, Weissman JS.   Functional Repurposing Revealed by Comparing S. pombe and S. cerevisiae Genetic Interactions.
Cell 2012 Jun 8;149(6):1339-52. [PubMed]

 

Joseph N, Hutterer A, Poser I, Mishima M.   ARF6 GTPase protects the post-mitotic midbody from 14-3-3-mediated disintegration.
EMBO J. 2012 May 11;31(11):2604-14. [PubMed]

 

Hegemann B, Hutchins JR, Hudecz O, Novatchkova M, Rameseder J, Sykora MM, Liu S, Mazanek M, Lénárt P, Hériché JK, Poser I, Kraut N, Hyman AA, Yaffe MB, Mechtler K, Peters JM.   Systematic phosphorylation analysis of human mitotic protein complexes.
Sci Signal. 2011 Nov 8;4(198):rs12. [PubMed]

 

Elefsinioti A, Saraç OS, Hegele A, Plake C, Hubner NC, Poser I, Sarov M, Hyman A, Mann M, Schroeder M, Stelzl U, Beyer A. Large-scale De Novo Prediction of Physical Protein-Protein Association.
Mol Cell Proteomics. 2011 Nov;10(11):M111.010629. [PubMed]

 

Ettinger AW, Wilsch-Bräuninger M, Marzesco AM, Bickle M, Lohmann A, Maliga Z, Karbanová J, Corbeil D, Hyman AA, Huttner WB.   Proliferating versus differentiating stem and cancer cells exhibit distinct midbody-release behaviour.
Nat Commun. 2011 Oct 18;2:503. [PubMed]

 

Ding L, Poser I, Paszkowski-Rogacz M, Buchholz F.   From RNAi Screens to Molecular Function in Embryonic Stem Cells.
Stem Cell Rev. 2011 Apr 28. [PubMed]

 

Jakobsen L, Vanselow K, Skogs M, Toyoda Y, Lundberg E, Poser I, Falkenby LG, Bennetzen M, Westendorf J, Nigg EA, Uhlen M, Hyman AA, Andersen JS. Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods.
EMBO J. 2011 Apr 20;30(8):1520-35. [PubMed]

 

Guizetti J, Schermelleh L, Mäntler J, Maar S, Poser I, Leonhardt H, Müller-Reichert T, Gerlich DW.
Cortical constriction during abscission involves helices of ESCRT-III-dependent filaments.
Science. 2011 Mar 25;331(6024):1616-20. [Article]

 

Schnütgen F, Ehrmann F, Poser I, Hubner NC, Hansen J, Floss T, deVries I, Wurst W, Hyman A, Mann M, von Melchner H. Resources for proteomics in mouse embryonic stem cells.
Nat Methods. 2011 Feb;8(2):103-4. [PubMed]

 

Huranová M, Ivani I, Benda A, Poser I, Brody Y, Hof M, Shav-Tal Y, Neugebauer KM, Stanek D.
The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells.
J Cell Biol. 2010 Oct 4;191(1):75-86. [PubMed]

 

Schmitz MH, Held M, Janssens V, Hutchins JR, Hudecz O, Ivanova E, Goris J, Trinkle-Mulcahy L, Lamond AI, Poser I, Hyman AA, Mechtler K, Peters JM, Gerlich DW. Live-cell imaging RNAi screen identifies PP2A-B55alpha and importin-beta1 as key mitotic exit regulators in human cells.
Nat Cell Biol. 2010 Sep;12(9):886-93. Epub 2010 Aug 15. [PubMed]

 

Vermeulen M, Eberl HC, Matarese F, Marks H, Denissov S, Butter F, Lee KK, Olsen JV, Hyman AA, Stunnenberg HG, Mann M. Quantitative Interaction Proteomics and Genome-wide Profiling of Epigenetic Histone Marks and Their Readers.
Cell. 2010 Sep 17;142(6):844-6. [PubMed]

 

Ankö ML, Morales L, Henry I, Beyer A, Neugebauer KM.  Global analysis reveals SRp20- and SRp75-specific mRNPs in cycling and neural cells.
Nat Struct Mol Biol. 2010 Aug;17(8):962-70. (Epub 2010 Jul 18) [PubMed]

 

Słabicki M, Theis M, Krastev DB, Samsonov S, Mundwiller E, Junqueira M, Paszkowski-Rogacz M, Teyra J, Heninger AK, Poser I, Prieur F, Truchetto J, Confavreux C, Marelli C, Durr A, Camdessanche JP, Brice A, Shevchenko A, Pisabarro MT, Stevanin G, Buchholz F.  A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia.
PLoS Biol. 2010 Jun 29;8(6). [PubMed]

 

Spruijt CG, Bartels SJ, Brinkman AB, Tjeertes JV, Poser I, Stunnenberg HG, Vermeulen M.
CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex.
Mol Biosyst. 2010 Sep;6(9):1700-6. (Epub 2010 Jun 4) [PubMed]

 

Hubner NC, Bird AW, Cox J, Splettstoesser B, Bandilla P, Poser I, Hyman AA, Mann M.   Quantitative proteomics combined with BAC TransgeneOmics reveals in vivo protein interactions.
J Cell Biol. 2010 May 17;189(4):739-54. [PubMed]

 

Neumann B, Walter T, Hériché JK, Bulkescher J, Erfle H, Conrad C, Rogers P, Poser I, Held M, Liebel U, Cetin C, Sieckmann F, Pau G, Kabbe R, Wünsche A, Satagopam V, Schmitz MH, Chapuis C, Gerlich DW, Schneider R, Eils R, Huber W, Peters JM, Hyman AA, Durbin R, Pepperkok R, Ellenberg J.  Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes.
Nature. 2010 Apr 1;464(7289):721-7. [PubMed]

 

Hutchins JR, Toyoda Y, Hegemann B, Poser I, Hériché JK, Sykora MM, Augsburg M, Hudecz O, Buschhorn BA, Bulkescher J, Conrad C, Comartin D, Schleiffer A, Sarov M, Pozniakovsky A, Slabicki MM, Schloissnig S, Steinmacher I, Leuschner M, Ssykor A, Lawo S, Pelletier L, Stark H, Nasmyth K, Ellenberg J, Durbin R, Buchholz F, Mechtler K, Hyman AA, Peters JM. Systematic Localization and Purification of Human Protein Complexes Identifies Chromosome Segregation Proteins.
Science. 2010 Apr 1. [PubMed]

 

Hübner NC, Wang LH, Kaulich M, Descombes P, Poser I, Nigg EA.
Re-examination of siRNA specificity questions role of PICH and Tao1 in the spindle checkpoint and identifies Mad2 as a sensitive target for small RNAs.
Chromosoma. 2010 Apr;119(2):149-65. (Epub 2009 Nov 11) [PubMed]

 

Lawo S, Bashkurov M, Mullin M, Ferreria MG, Kittler R, Habermann B, Tagliaferro A, Poser I, Hutchins JR, Hegemann B, Pinchev D, Buchholz F, Peters JM, Hyman AA, Gingras AC, Pelletier L.  HAUS, the 8-subunit human Augmin complex, regulates centrosome and spindle integrity.
Curr Biol. 2009 May 26;19(10):816-26.  [PubMed]

 

Sapra AK, Ankö ML, Grishina I, Lorenz M, Pabis M, Poser I, Rollins J, Weiland EM, Neugebauer KM.
SR protein family members display diverse activities in the formation of nascent and mature mRNPs in vivo.
Mol Cell. 2009 Apr 24;34(2):179-90. [PubMed]

 

Theis M, Slabicki M, Junqueira M, Paszkowski-Rogacz M, Sontheimer J, Kittler R, Heninger AK, Glatter T, Kruusmaa K, Poser I, Hyman AA, Pisabarro MT, Gstaiger M, Aebersold R, Shevchenko A, Buchholz F.   Comparative profiling identifies C13orf3 as a component of the Ska complex required for mammalian cell division.
EMBO J. 2009 May 20;28(10):1453-65. [PubMed]

 

Ding L, Paszkowski-Rogacz M, Nitzsche A, Slabicki MM, Heninger AK, de Vries I, Kittler R, Junqueira M, Shevchenko A, Schulz H, Hubner N, Doss MX, Sachinidis A, Hescheler J, Iacone R, Anastassiadis K, Stewart AF, Pisabarro MT, Caldarelli A, Poser I, Theis M, Buchholz F.   A genome-scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity.
Cell Stem Cell. 2009 May 8;4(5):403-15. (Epub 2009 Apr 2) [PubMed]

Temperature

Temperature

 

Leaver M, Kienle S, Begasse ML, Sommer RJ, Hyman AA. A locus in Pristionchus pacificus that is responsible for the ability to give rise to fertile offspring at higher temperatures.
Biol Open. 2016 Jul 18. pii: bio.018127. doi: 10.1242/bio.018127. [FullText] [Video Abstract]

 

Begasse ML, Leaver M, Grill SW, Hyman AA. Temperature dependence of cell division timing accounts for a shift in the thermal limits of C. elegans and C. briggsae.
Cell Reports. 2015; 10:1-7. [FullText] Watch Maria’s video abstract for this paper!

 

Begasse ML and Hyman AA. The first cell cycle of the Caenorhabditis elegans embryo: spatial and temporal control of an asymmetric cell division.
Results Probl Cell Differ. 2011;53:109-33. [PubMed]

Other

Other

 

Mahamid J, Pfeffer S, Schaffer M, Villa E, Danev R, Cuellar LK, Förster F, Hyman AA, Plitzko JM, Baumeister W. Visualizing the molecular sociology at the HeLa cell nuclear periphery.
Science. 2016 Feb; 351(6276):969-72. [PubMed]

 

Arnold J, Mahamid J, Lucic V, de Marco A, Fernandez J, Laugks T, Mayer T, Hyman AA, Baumeister W, Plitzko JM. Site-specific cryo-focused ion beam sample preparation guided by 3D correlative microscopy.
Biophysical Journal. 2016 Feb; 110(4):1-10. [PubMed]

 

Toyoda Y, Erkut C, Pan-Montojo F, Boland S, Stewart MP, Müller DJ, Wurst W, Hyman AA, Kurzchalia T. Products of the Parkinson’s disease-related glyoxalase DJ-1, D-lactate and glycolate, support mitochondrial membrane potential and neuronal survival.
Biol Open. 2014 Jul 25. pii: BIO20149399. [FullText]

 

El-Labban A, Zisserman A, Toyoda Y, Bird AW, Hyman A. Temporal models for mitotic phase labelling.
Med Image Anal. 2014 May 22;18(7):977-988. [PubMed]

 

Redemann S, Schloissnig S, Ernst S, Pozniakowsky A, Ayloo S, Hyman AA, Bringmann H.  Codon adaptation-based control of protein expression in C. elegans.
Nat Methods. 2011 Mar;8(3):250-2. (Epub 2011 Jan 30)  [PubMed]

 

Pozniakovsky AI, Knorre DA, Markova OV, Hyman AA, Skulachev VP, Severin FF.  Role of mitochondria in the pheromone- and amiodarone-induced programmed death of yeast.
J Cell Biol. 2005 Jan 17;168(2):257-69.  [PubMed]