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Contents

General
Affinity purification of antisera on antigen
Ammonium sulfate precipitation
Antigen design and administration
BAC Modification (GeneBridges, with Tips from Alex B)
Counter-Selection BAC Modification (GeneBridges, with Tips from Alex B)
DMSO-Competent E. coli cells
DNA precipitation
Electrocompetent Bacteria
General GST expression
Immunization and Antibody Purification Protocols
Removing HSPs bound to E. coli fusion proteins
Silver staining
Standard Blotting Procedure
TCA Precipitation of Proteins
The Slacker (Though Kit-Free) Way for Making Fusion Proteins for Antibody Generation/Affinity Purification
Transformation of DMSO-Competent E. coli
How To Make
Actin Prep from Brains
Antibiotic Concentrations (Sigma recommendations) (pdf)
Antibiotic Concentrations (ATCC recommendations) (pdf)
Biotinylation of Smooth Muscle Myosin
Coupling IgG to Affiprep Beads
Expression, purification, denaturation, and renaturation of T7Δ90
Making dialysis tubing
Making p50 Dynamitin
NHS-rhodamine labelling of actin
Recombinant p50/dynamitin [Meth Cell Biol 61] (pdf)
Tubulin
Centrosome Prep
CHO Centrosome Prep
Labeling Tubulin and Quantifying Labeling Stoichiometry
Tubulin Prep (shortened)
Tubulin Preparation from Bovine Brain
Tubulin Preparation from Porcine Brain
Tubulin Protocols (Mitchison Lab)
Xenopus
Frog Colony Rules
Microtubule dynamics by fluorescence microscopy in Xenopus egg extracts
Preparation of DNA beads
Preparation of Xenopus laevis egg extracts
Spindle assembly in vitro
Methods for the study of centrosome-independent spindle assembly in Xenopus egg extracts [Meth Cell Biol 67] (pdf)
C. elegans
Biolistic transformation of c.elegans (bombardment protocol)
C. elegans biochemistry
C. elegans dissection for embryo analysis
C. elegans techniques - Basics
C. elegans techniques - Immunofluorescence and Microscopy
C. elegans techniques - Protein Biochemistry
Cenix protocol for production of large scale dsRNA
dsRNA production for RNAi
Growing worms on plates
Immunostaining
Making agarose pads
RNAi by feeding
RNAi by feeding 2
RNAi by soaking
Setup and adjustment of the lasercutter
Whole-worm immunoprecipitation