The goal of this project is to establish a genome-wide resource of GFP-tagged genes expressed in BACs (bacterial artificial chromosomes). The advantage of using BACs is that the expression of the tagged gene is controlled by its own promoter. For this purpose, a new human BAC library is currently produced. Next, the TransgeneOmics Facility (MPI-CBG) headed by Mihail Sarov will tag all genes that are in BACs using BAC recombineering in high-throughput. All tagged BACs will be transfected into eukaryotic cells using the new cell culture robot build within the “Autranomics” project.
Our aim was to test all C. elegans genes for their role in the first two rounds of mitotic cell division. To this end, we combined genome-wide RNAi screening with time-lapse video microscopy of the early embryo.
The goal of this project was to conduct a functional genomic screen to identify genes required for cell division processes in metazoans. The early C. elegans embryo was chosen as a model system for the following reasons. First, cell division processes can be examined with high spatial and temporal resolution using simple time-lapse differential interference contrast (DIC) microscopy. Second, the C. elegans genome sequence is available. Third, using RNA interference (RNAi), the expression of a given gene in the embryo can be abolished in a sequence-specific manner by exposing parental germ cells to corresponding double-stranded (ds) RNA. Thus, RNAi was applied on a genomic scale (starting with chromosome III) to identify genes required for cell division processes.
This contains a set of C.elegans RNAi movies recorded in various GFP strains.
MitoCheck is an integrated research project which brought together leading European research groups to study systematically the regulation of mitosis in human cells. It ran from 2004-2009.
The MitoSys (systems biology of mitosis) project (2010-2015) will take on this challenge to tackle mitosis from a systems biology perspective. Internationally leading biologists, mathematicians, biochemists/ biophysicists working at thirteen research institutes, universities, international organizations and companies in eight different European countries will collaborate on this project to reveal how genes and proteins orchestrate mitosis in human cells. MitoSys will receive ten million Euro from the European Union under its seventh framework programme (FP7).
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